Acid Deposition in the Tuscan Appennines During the Dry Period 1988-89

The typical parameters of acid precipitation are evaluated in the forest of Vallombrosa (Tuscan Appennines) during the dry period 1988-89. Individual rain events (dry and wet deposition) were sampled in a clearing of the forest and below the canopy of an evergreen tree as well as a deciduous broadleaf tree.

In atmospheric precipitation the pH values usually vary around 4.4, with neutralization in the hot season due to calcareous material from distant sources. Relatively large concentrations of Pb and Cd are found in rain, but only in a small amount in canopy leachate. Aluminium, manganese and iron are more significantly washed off than Pb and Cd.     

Trophoblast-like cells sorted from peripheral maternal blood using flow cytometry: A multiparametric study involving transmission electron microscopy and fetal dna amplification

Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities.     

Human induced salinisation and the use of quantitative methods

Sustainable development of the earth's limited water and land resources is of paramount importance because of rising world population and existing conflicting demands for these resources. Enormous capital investment has been made in developing these resources, but now there is irrefutable evidence that such developments have led to major resource degradation. This includes problems of salinisation and damages to ecosystems. The countries predominantly affected by human induced salinisation are located in arid and semi-arid regions of the world and include Australia, China, Egypt, India, Pakistan, USSR, and USA. This paper describes the processes of salinisation, its impacts and the use of quantitative methods in salinity investigation and management. Australia is used as a case study of typical salinity problems and as a demonstration of the fruitful application of quantitative methods. The paper concludes that quantitative methods such as surface water and groundwater models are powerful design, management and predictive tools in salinity investigation. However, application of some models, such as those for unsaturated flow and transport and groundwater solute transport, are not widespread due to uncertainties in describing the complexity of the processes and the lack of hydrodispersive data.     

Prenatal diagnosis of femur-fibula-ulna complex by ultrasonography in a male fetus at 24 weeks of gestation

We present a case of ultrasonographic prenatal diagnosis at 24 weeks of femur—fibula—ulna (FFU) complex. To our knowledge, this is the first report of an early prenatal diagnosis of FFU.     

In situ assessment of modification of sediment properties by burrowing invertebrates

Benthic organisms can significantly alter the physical properties of marine sediments, but it has hitherto been difficult to assess and quantify the effects of bioturbation. In situ geophysical techniques offer new methods for measuring these effects: measurement of acoustic shear-wave velocity and electrical resistivity allows nondestructive assessment of the properties of the grain framework and pore-fluid matrix, respectively, of the seabed sediment. The influence of burrowing invertebrates on the structural properties of sandy sediments at intertidal locations on the coast of Wales (UK) was investigated during the periol 1986–1987 using these techniques. Three species (Arenicola marina, Corophium arenarium and Lanice conchilega) were selected on the basis of their contrasting styles of burrow construction. All three species produced measurable and significant, although different, changes in bed properties. They modified shear-wave propagation through the bed by changing bed rigidity: while A. marina and C. arenarium decreased rigidity by creating open burrows, L. conchilega increased rigidity by building shell-lined tubes. All produced a decrease in electrical resistivity by altering porosity and/or tortuosity, which implies an increase in permeability; these changes were attributable not only to the presence of the burrows but also to modification of the between-burrow sediment texture and bed properties.     

Exposure to radiation from global system for mobile communications at 1,800 MHz significantly changes gene expression in rat hippocampus and cortex

We have earlier shown that radio frequency electromagnetic fields can cause significant leakage of albumin through the blood–brain barrier of exposed rats as compared to non-exposed rats, and also significant neuronal damage in rat brains several weeks after a 2 h exposure to a mobile phone, at 915 MHz with a global system for mobile communications (GSM) frequency modulation, at whole-body specific absorption rate values (SAR) of 200, 20, 2, and 0.2 mW/kg. We have now studied whether 6 h of exposure to the radiation from a GSM mobile test phone at 1,800 MHz (at a whole-body SAR-value of 13 mW/kg, corresponding to a brain SAR-value of 30 mW/kg) has an effect upon the gene expression pattern in rat brain cortex and hippocampus—areas where we have observed albumin leakage from capillaries into neurons and neuronal damage. Microarray analysis of 31,099 rat genes, including splicing variants, was performed in cortex and hippocampus of 8 Fischer 344 rats, 4 animals exposed to global system for mobile communications electromagnetic fields for 6 h in an anechoic chamber, one rat at a time, and 4 controls kept as long in the same anechoic chamber without exposure, also in this case one rat at a time. Gene ontology analysis (using the gene ontology categories biological processes, molecular functions, and cell components) of the differentially expressed genes of the exposed animals versus the control group revealed the following highly significant altered gene categories in both cortex and hippocampus: extracellular region, signal transducer activity, intrinsic to membrane, and integral to membrane. The fact that most of these categories are connected with membrane functions may have a relation to our earlier observation of albumin transport through brain capillaries.     

Toxic equivalent concentrations (TEQs) for baseline toxicity and specific modes of action as a tool to improve interpretation of ecotoxicity testing of environmental samples

The toxic equivalency concept is a widely applied method to express the toxicity of complex mixtures of compounds that act via receptor-mediated mechanisms such as induction of the arylhydrocarbon or estrogen receptors. Here we propose to extend this concept to baseline toxicity, using the bioluminescence inhibition test with Vibrio fischeri, and an integrative ecotoxicity endpoint, algal growth rate inhibition. Both bioassays were validated by comparison with literature data and quantitative structure-activity relationships (QSARs) for baseline toxicity were developed for all endpoints. The novel combined algae test, with Pseudokirchneriella subcapitata, allows for the simultaneous evaluation of specific inhibition of photosynthesis and growth rate. The contributions of specific inhibition of photosynthesis and non-specific toxicity could be differentiated by comparing the time and endpoint pattern. Photosynthesis efficiency, measured with the saturation pulse method after 2 h of incubation, served as indicator of specific inhibition of photosynthesis by photosystem II inhibitors. Diuron equivalents were defined as toxicity equivalents for this effect. The endpoint of growth rate over 24 h served to derive baseline toxicity equivalent concentrations (baseline-TEQ). By performing binary mixture experiments with reference compounds and complex environmental samples from a sewage treatment plant and a river, the TEQ concept was validated. The proposed method allows for easier interpretation and communication of effect-based water quality monitoring data and provides a basis for comparative analysis with chemical analytical monitoring.